ABSTRACT
Background: Cyclophosphamide (CP), [2-(bis-/2-chloro-ethyl-amino)-tetrahydro-2H-1, 2, 3 oxazaphosphorine-2-oxide], is an alkylating chemotherapeutic agent, which is commonly used against malignancies, such as leukemia, lymphoma, breast, lung, prostrate, and ovarian cancer. The aim of this study is to evaluate the side effect of CP on male albino rat in response to certain hematological and hepatic and renal biochemical parameters. Methods: In this investigation, total 20 albino rats were divided in to two groups of ten each. Group first served as control received i.p. injection of 0.9 physiological saline fed with standard food and water ad libitum. While, Group second received a single dose (0.4ml/100g b/w) through i.p. injection of cyclophosphamide once in a week for a period of 7 and 35 days and hematological parameters i.e. Hb%, total count-RBCs, total count-WBCs along with some biochemical estimations i.e. protein and creatinine levels in liver and kidney were quantified after 7 and 35 days of the treatments. Results: The hematological parameters i.e. Hb%, total count-RBCs, total count –WBCs and protein and creatinine levels in liver and kidney were significantly lowered after 7 and 35 days treatment of cyclophosphamide. The lowering of these values was more prominent in later part of the experiment. Conclusion: Alteration in hematological parameters after cyclophosphamide exposure may impair the functional activities of liver and kidney of male Rattus norvegicus by interfering enzymatic metabolic activities and protein synthesis.
ABSTRACT
The present investigation is aiming at studying the effect of oral administration of aspirin (acetylsalicylic acid, ASA) drug on female albino rat, Rattus rattus norvegicus. The female rats (n=24) were allocated into 2 groups as control and treated. The treated rats (n=12) were given orally with high dose of aspirin at a dose of 100 mg/ kg body weight for 15 days (n=6) and 30 days (n=6). And body weight, relative organ weights, hematological parameters such as Hb %, total count RBC and total count WBC; and biochemical estimations in blood serum such as glucose, protein, cholesterol and calcium were measured. The aspirin did not showed much significant variations in the body weight, relative organ weights, serum protein, serum cholesterol, serum calcium, total counts RBC and Hb %; while, aspirin declined serum glucose level and elevated total count of WBC. The present investigation indicated that the aspirin impairs serum glucose level and total count of WBC by modulating of certain enzymes metabolism in albino rats.